维药材小豆蔻提取物的体外抑菌实验研究

Study on the Antibacterial Effect of Extracts from Elettaria Cardamomum in Vitro

采用微孔板法和稀释法研究小豆蔻不同极性提取物对变异链球菌和大肠杆菌的体外抑菌作用。结果显示小豆蔻的氯仿、乙酸乙酯提取物对变异链球菌的生长有部分抑制作用,正丁醇、乙醇和水提取物对变异链球菌生物膜形成有部分抑制作用;小豆蔻的不同极性提取物对大肠杆菌生物膜形成均有一定抑制作用,其中氯仿、乙酸乙酯和正丁醇提取物的抑制活性最强。小豆蔻中含有抑制供试菌生物膜形成的活性成分,值得进一步研究。

To study the antibacterial activities of the different solvent extracts from Elettaria cardamomum. Microwell plate method and double dilution method were used to investigate the antibacterial effect of the different solvent extracts from Elettaria cardamomum on streptococcus mutans and Escherichia coli in vitro. The results showed that the chloroform extract and ethyl acetate extract has certain inhibition on the growth of s tre top coccus mutaus and the n-butanol extract, ethanol extract and water extract has partial inhibition on the biofilm formation of stretopcoccus mutans. The different solvent extracts all could inhibit the formation of biofilm of Escherichia coil,especially chloroform extract, ethyl acetate extract and n-butanol extract showed the best antibacterial activity. The extracts of Elettaria cardamomum showed obvious antibacterial activity ,and were worth further studying.