LY294002联合顺铂对脑胶质瘤U87细胞株增殖的影响

Effect of LY294002 combined with CDDP on proliferation in glioma U87 cells in vitro

目的体外观察磷酸肌醇-3激酶(PI3K)特异性抑制剂LY294002与顺铂(CDDP)联合对人脑胶质瘤U87细胞株增殖的抑制作用。方法采用细胞毒实验(MTT)法、流式细胞术(FCM)检测LY294002单独或联合CDDP对人脑胶质瘤U87细胞增殖的抑制作用,并观察细胞形态学变化。结果 LY294002与CDDP均能抑制U87细胞生长,呈剂量依赖性,两者联合有协同效应。当CDDP为0.625μg/mL和LY294002为5μmol/L时达到最大协同作用(Q=1.21),FCM检测细胞被阻滞在G1期,S期细胞比例减少,差异有统计学意义(P<0.01)。结论 LY294002能有效提高神经胶质瘤U87细胞对CDDP的敏感性,本研究为两种化疗药物在临床上的联合应用提供了理论依据。

Objective To investigate the effects of LY294002 and CDDPiamycin（CDDP） on proliferation of glioma cancer cell line U87.Methods MTT assay was used to examine the inhibition rate cell growth when cells were treated at various concentrations of LY294002 and CDDP alone or combination.Cell cycle and apoptosis rate of U87 cells were detected by flow cytometry（FCM） after using drugs.Optic microscope was used to detect the morphological changes of U87 cells.Results Both LY294002 and CDDP exhibited a dose dependent inhibitory effect on the proliferation of U87 cells.The combination of LY294002 and CDDP exerted a synergistic effect on U87 cell growth inhibition.LY294002 in the concentration of 5 μmol/L and CDDP in the concentration of 0.625 μg/mL interaction was the most synergistic（Q=1.21）.Combined treatment group raised U87 cells′apoptosis and induced G1 phase arrest compare with single drug treatment group（P0.01）.Conclusion LY294002 could effectively induce U87 sensitivity to CDDP.And this conclusion provides theoretical basis for the conbined clinical use of LY294002 and CDDP.