外周血双阴性T细胞升高与临床疾病的关系

The relationship between the increase of double negative T cell in peripheral blood and clinical disease

目的了解外周血双阴性T细胞(DNT)含量升高的临床疾病谱,探讨DNT升高的临床意义。方法回顾性分析2010年1月至2012年2月,收治患者中接受外周血T淋巴细胞亚群检测且DNT升高的病历资料,以临床诊断为金标准,按照疾病分类统计分析。T淋巴细胞亚群检测采用流式细胞学检测技术。结果健康人外周血DNT占淋巴细胞的(7.36±2.13)%,含量达(0.13±0.06)×109/L。送检患者中约有5.45%的外周血DNT增高,主要见于肺部细菌感染、全身性病毒感染、不明原因发热、胸膜炎、急性扁桃体炎、创伤、脑膜炎等。DNT在外周血淋巴细胞中的比例增高和绝对含量增高均以急性扁桃体炎为最高,不明原因的发热次之。增高患者中又以13～17岁青少年患者DNT升幅为最大。结论外周血DNT主要是在临床急性炎症性疾病时明显升高,可能参与了急性感染、严重创伤等患者的细胞免疫调节。

Objective To analyze the clinical spectrums of disease of abnormal elevation of double negative T cells in peripheral blood,and identify the clinical significance of double negative T cells（DNT） increase.Methods We did a retrospective analysis of the medical data of patients who were DNT increased in peripheral blood obtained from January 2010 to February 2012.Our research set clinical diagnosis as gold standard and we conducted the statistical analysis in accordance with the classification of diseases.T lymphocyte subsets were assessed by flow cytometry.Results The DNT percentage and absolute quantity was（7.36±2.13）% and（0.13±0.06）×109/L in healthy people.There were about 5.45% patients had an increased DNT levels in all delivered T cell subset examination patients.DNT increasing in peripheral blood mainly found in pneumonic bacterial infection,general viral infection,innubibus fever,pleuritis,acute tonsillitis,trauma and cephalomeningitis,etc.Acute tonsillitis patients had the highest percentage and absolute quantity increase of DNT,followed by innubibus fever.In addition,adolescents aged 13 to 17 had the highest increase of DNT.Conclusion DNT were significantly increased in peripheral blood of acute inflammation patients,and it maybe take part in regulating cell immunity in patients with acute infection,severe trauma,etc.