某县204例乙型肝炎病毒基因型及其临床相关性分析

Analysis on 204 cases of hepatitis B virus genotypes in a county and their clinical correlation

目的了解重庆垫江县地区乙型肝炎病毒(HBV)基因型的分布及其与临床的相关性。方法选取垫江县HBV-DNA阳性的HBV感染者204例,应用聚合酶链反应(PCR)和直接测序法检测HBV基因型并分析其与临床的相关性。结果 204例HBV-DNA阳性血清标本中,测序成功为146例。其中B型98例(67.12%),C型47例(32.19%),D型1例(0.69%),C基因型丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)指标高于B基因型(P<0.05);C基因型患肝硬化和肝细胞癌的比例高于B基因型(P<0.05)。结论 B型为本地区的优势基因型,并且C型HBV感染者易引起肝脏的严重损伤。

Objective To investigate the distribution of hepatitis B virus（HBV） genotypes in Dianjiang area of Chongqing and their clinical correlation.Methods Serum samples from 204 HBV-DNA positive patients with chronic HBV infection,including 183 cases of chronic hepatitis B（CHB）,15 cases of liver cirrhosis（LC） and 6 cases of hepatocellular carcinoma（HCC）,were collected and detected for HBV genotypes by PCR and direct sequencing.Their clinical correlation was analyzed.Results Of all 204 HBV-DNA positive serum samples,146 cases were detected successfully by direct sequencing,including 98 cases（67.12%） of genotype B,47 cases（32.19%） of genotype C and only 1 cases（0.69%） of genotype D.The value of ALT and AST in genotype C was significantly higher than that in genotype B（P0.05）;genotype C was more prevalent in LC and HCC than genotype B（P0.05）.Conclusion Genotype B is the superior genotype in this area.Genotype C may induce more serious damage of liver in the patients with chronic HBV infection.