摘要
目的分析miRNA-20a在大肠癌中的表达及其与临床病理特征的关系。方法选择52例肠癌患者的手术标本(癌组织及其癌旁组织)提取总RNA,采用TaqMan探针逆转、实时定量PCR检测miRNA-20a在大肠癌及其癌旁正常组织中的表达量,并分析其与临床病理特征关系。结果肠癌组织miRNA-20a表达量为2.05(0.046~10.274),与癌旁正常组织相比显著上调(Z=-3.597,P=0.000);miRNA-20a的表达与TNM分期(T=3.223,P=0.001)相关,TNM分期越高,miRNA-20a表达越高;miRNA-20a也与淋巴结转移相关(Z=-2.141,P=0.037),淋巴结转移患者miRNA-20a表达增加。miRNA-20a表达与性别、年龄、肿瘤位置和组织分化无关。结论 miRNA-20a表达上调可能与大肠癌的发生及其转移有关。
Objective To investigate the expression of miRNA-20a in colorectal cancer and its relation with clinical pathology features.Methods Total RNA were extracted from 52 paired colorectal cancer(CRC) tissues and para cancer normal tissues which were excised surgically.miRNA-20a was detected by real time PCR with TaqMan probe.Then,the clinical significance of miRNA-20a was analyzed.Results The expression level of miRNA-20a in CRC tissues was 2.05(0.046-10.274),which was significantly higher than para cancer normal tissues(Z=-3.597,P=0.000);and the expression of miRNA-20a was correlated with TNM staging(T=3.223,P=0.001),tissues with higher TNM stages expressed higher miRNA-20a.miRNA-20a levels were also related to lymph node invasion(Z=-2.141,P=0.037),tissues with positive node invasion expressed higher miRNA-20a.No significant correlation was found between miRNA-20a and gender,age,tumor site and tissue differentiation.Conclusion The upregulation of miRNA-20a might play a role in tumorigenesis and metastasis of colorectal cancer.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第36期3809-3811,共3页
Chongqing medicine