Slug基因在宫颈癌、宫颈上皮内瘤变组织中的表达与意义

Expression and clinical significance of Slug gene in cervical cancer and cervical intraepithelial neoplasia

目的研究转录因子Slug mRNA在宫颈癌、宫颈上皮内瘤变(CIN)组织中的表达情况及意义。方法采用实时定量PCR方法检测56例宫颈癌组织、60例CIN组织中Slug mRNA的相对表达量,以35例正常宫颈组织及慢性宫颈炎组织作为对照,并分析其与宫颈癌各临床病理参数的相关性。结果对照组、CIN组、宫颈癌组Slug mRNA相对表达水平分别为(1.33±0.32)×10-2、(2.99±0.95)×10-2、(4.69±1.02)×10-2,宫颈癌组、CIN组Slug mRNA相对表达水平高于对照组,差异有统计学意义(P<0.01);宫颈癌组Slug mRNA相对表达水平高于CIN组,差异有统计学意义(P<0.01)。宫颈癌组织中Slug的表达与国际妇产科学联盟(FIGO)分期、淋巴结转移相关,差异有统计学意义(P<0.05),但与年龄、肿瘤大小、病理类型、分化程度无关,差异无统计学意义(P>0.05)。结论 Slug基因在CIN、宫颈癌组织中表达上调,可能与宫颈癌的发生、发展及转移密切相关。

Objective To investigate the expression pattern and significance of transcription factor Slug in CIN and cervical cancer tissues.Methods The expression of Slug mRNA in tissues of 56 cases of cervical cancer,60 cases of CIN,35 cases of normal cervical or chronic cervicitis（control group） was detected by Real-time PCR.The correlation analysis was conducted to assess the relationship between Slug expression and clinical pathological parameters of cervical cancer.Results The level of Slug mRNA expression in control group,CIN group and cervical cancer group was（1.33±0.32）×10-2,（2.99±0.95）×10-2,（4.69±1.02）×10-2,respectively,the expression of Slug in cervical cancer group and CIN group was significantly higher than that in control group（P0.01）,the expression of Slug in cervical cancer group was significantly higher than that in CIN group（P0.01）,Slug expression in the cervical cancer tissues was correlated to FIGO stage and lymph node metastasis（P0.05）,but not to age,tumor size,differentiation status of tumor and pathological type（P0.05）.Conclusion The up-regulated expressions of Slug gene in the CIN and cervical cancer tissues are probably associated with the initiation,progression and metastasis of cervical cancer.