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IL-3Rα在急性白血病中的表达及意义

The expression of IL-3Rα in acute leukemia and its clinical significance
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摘要 目的探讨白细胞介素-3受体α亚基(IL-3Rα)在急性白血病(AL)中的表达及意义。方法建立AL患者标本,采用逆转录-聚合酶链反应(RT-PCR)方法检测IL-3Rα的表达,分别予以观察IL-3RαmRNA表达与AL和CD34的相关性。结果对照组未检测到IL-3Rα表达;AML和B-ALL患者IL-3Rα阳性表达显著高于对照组,T-ALL患者未检测到IL-3Rα的表达,M1～M5各型患者的阳性率差异无统计学意义(P>0.05);AML和B-ALL患者的IL-3Rα阳性表达与CD34显著相关,差异有统计学意义(P<0.05);患者中IL-3Rα阳性表达的患者CR率低于阴性者,且与外周血白细胞计数、骨髓中原始细胞比例有关。结论 IL-3Rα在AML和B-ALL发病中有一定作用,检测IL-3Rα有助于ALL的T和B两型的鉴别。IL-3Rα基因可能是AML、B-ALL的一个不良预后因素。 Objective To evaluate the expression of IL-3Rα and its clinical significance in acute leukemia（AL） patients.Methods The AL specimens of patients was established.The expression of IL-3Rα mRNA in bone marrow or peripheral blood mononuclear cells from 94 AL patients were detected by RT-PCR method.The correlation of IL-3R αmRNA expression with AL and CD34 were observed respectively.Results 10 healthy subjects didn′t express IL-3Rα mRNA.The expression of IL-3Rα in AML and B-ALL patients were higher than that in control group.IL-3Rα mRNA didn′t express in T-ALL.IL-3Rα mRNA was expressed in all AML subtypes,and there was no significant difference between AML subtypes（P0.05）.The expression of IL-3Rα in AML and B-ALL was significantly correlated with CD34 positive expression（P0.05）.The CR rate of IL-3Rα＋ cases was lower than that of IL-3Rα（-） cases,and the expression of IL-3Rα mRNA related to white blood count and blast cells ratio.Conclusion IL-3Rα gene might play an important role in the pathogenesis of AML and B-ALL.It might be helpful to distinguish B-ALL from T-ALL.IL-3Rα gene might convey adverse prognosis of AML and B-ALL.
作者 彭鹏 宋玉华
机构地区 东南大学医学院附属南京市第二医院肿瘤科
出处 《重庆医学》 CAS CSCD 北大核心 2012年第36期3840-3842,共3页 Chongqing medicine
关键词 白细胞介素3受体α亚单位 急性白血病 基因 interleukin-3 receptor alpha subunit acute leukemia gene
分类号 R733.71 [医药卫生—肿瘤]
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参考文献11

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重庆医学
2012年 第36期

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