马玲薯葡萄糖培养基在延胡索酸泰妙菌素菌种选育中的应用

Application of Potato Dextrose Culture Medium in Selection and Breeding of Strains of Tiamulin Fumarate

采用涂布法将延胡索酸泰妙菌素生产菌种TMⅡ10-444分离于不同平板培养基上(生产用麦芽浸膏培养基和马铃薯葡萄糖培养基),置恒温恒湿培养箱中培养成熟后,挑取单菌落、初筛、复筛,采用高效液相色谱(HPLC)法测定效价。结果表明,两种不同平板培养基上的菌落生长有差异,马铃薯葡萄糖培养基上的菌落比对照培养基上的菌落明显长得快;对马铃薯葡萄糖培养基选育的菌株进行了筛选,最终获得4支高于对照生产菌株,分别是2107、2115、2165和2183。此结论为延胡索酸泰妙菌素产生菌菌种选育提供了一种新方法。

The production strain of TMII10 -444. tiamulin fumarate was coated using coating method separately in two different plating medium, i.e. malt extract medium （MEM） and potato dextrose agar medium （PDAM）, under the craft conditions of constant temperature and humidity in the culture box until maturation. Then picking out a single colony, screening, and re - screening. HPLC method was used to measure chemical titer. There was difference of plate colony growth in observed two different culture mediums. The colony on PDAM grew obviously faster than that on MEM. The breeding strains were screened and selected on PDAM. There were four strains eventually selected out by comparing their higher yield titers. These four strains were 2107, 2115, 2165, and 2183, respectively.