盘基网柄菌KAx-3中类免疫细胞的初步研究

Preliminary study on the immune-like cells during Dictysotelium discoideum development

以Ethidium bromide(EB)代替盘基网柄菌生长环境中所遇到的毒物,检测盘基网柄菌中是否存在能吞噬毒物的特定细胞.荧光显微镜下观察,细胞丘时期仅能看到EB加入后显现的暗红色轮廓;Hoechst33342定位细胞核,以确证EB被吞噬进细胞内;蛞蝓体时期少数细胞有明显的强荧光反应,随着蛞蝓体的爬行,含EB的细胞团被丢弃在遗弃的粘液鞘中.表明多细胞发育至蛞蝓体阶段存在一种能吞噬EB的细胞,最终将EB排出体外.流式细胞术检测发现,该类特殊的细胞主要来自于前柄细胞.结果显示,盘基网柄菌中存在简单的内在免疫细胞,为研究免疫系统在生物体的发生和发展提供了研究基础.

To study the immune-like cells during the development of Dictyostelium discoideum,fluorescent dye Ethidium bromide（EB） was used as surrogates for the environment toxins that Dictyostelium might encounter in the soil.With the help of fluorescence microscopy,only rufous mound cells were visualized.Positioning of nucleus by Hoechst33342 was to confirm that EB was phagocytosed into the intracellular.During the slug stage,the florescent dye EB displayed highly accumulation within a small subset cells and eventually was found in the abandoned sheath as the slug continued to migrate.The special kind cells in the slug had the ability to sequester EB.The results from flow cytometry showed that the immune-like cells existed in pre-stalk cells,suggesting the simple immune-like cells present in Dictyostelium discoideum,which is the foundation to futher study the occurrence and development of immunity system.