p16基因在尖锐湿疣及其癌变中的意义

Significance of p16 Gene in Condyloma Acuminatum

目的 探讨 p16基因在尖锐湿疣及其癌变中的作用及临床意义。方法 应用聚合酶链－单链构象多态性分析（ PCR－ SSCP）检测 33例尖锐湿疣和 7例尖锐湿疣癌变患者 p16基因有无纯合性缺失和点突变。结果 p16基因在尖锐湿疣及其癌变中存在纯合性缺失，而且缺失程度在尖锐湿疣癌变中明显升高，且是尖锐湿疣癌变的早期表现；未发现 p16基因点突变。结论 尖锐湿疣及其癌变与抑癌基因 p16失活有关，临床上通过对尖锐湿疣患者 p16基因的检测，有可能对尖锐湿疣癌变作出可能性预测及早期诊断，为发现癌变高危人群提供新的依据。

Objective To investigate the clinical significance of p16 gene in condyloma acuminatum (CA) and the role in its canceration. Methods Both homologous deletion and mutation of p16 gene were tested in 33 CA samples and 7 samples of CA with canceration by polymerase chain reaction－ single strand conformation polymorphism（ PCR－ SSCP） .Results The homologous deletion of p16 gene occurred in CA and CA with canceration. The deletion rate of p16 gene increased significantly in CA with canceration, and homologous deletion of p16 gene was found in the early stage of CA with canceration. There was no mutation of p16 gene in CA and its canceration. Conclusion The change of p16 gene is related to CA and its canceration. Detecting p16 gene in CA patients might be used to find high risk canceration group.