生殖道沙眼衣原体细胞培养和血清学研究

Detection of Genital C. trachomatis Infection by Cell Culture and Serology

目的 研究孕妇、盆腔炎患者和 STD门诊男女就诊者生殖道沙眼衣原体病原学、血清学及两者之间的关系，并与对照人群进行比较。 方法 细胞培养检测生殖道沙眼衣原体；酶免疫和微量免疫荧光试验方法检测血清沙眼衣原体抗体。结果 孕妇、盆腔炎患者和 STD男女就诊者生殖道沙眼衣原体阳性率分别为 3.3％ ,6.7％ ,20.4％和 15.6％；血清沙眼衣原体酶免疫法检测 IgG/微量免疫荧光试验方法 IgG抗体阳性率分别 46.7％ /11.9％ ,70.2％ /39.9％ , 73.5％ /52.4％和 75.4％ /63.1％，统计学上均极为显著高于对照人群 (孕妇组除外 )。女性生殖道沙眼衣原体阳性者比阴性者统计学上具有更高的血清沙眼衣原体抗体，男性则无这种差异。 8例存在沙眼衣原体急性抗体。结论 应常规筛查 PID患者沙眼衣原体，但尚无需筛选孕妇 ;女性宫颈沙眼衣原体感染与血清学具有重要的关联 ;血清学无助于生殖道沙眼衣原体感染的诊断。

Objectives To determine the prevalences of genital C. trachomatis and serum chlamydial antibodies in pregnant women, PID patients and the patients attending STD clinic, and to compare the chlamydial serology with control subjects. Methods Genital C. trachomatis was detected by cell culture, and serum chlamydial antibodies were measured by EIA and MIF. Results Genital C. trachomatis was found in 3.3％ of pregnant women, 6.7％ of PID patients, 20.4％ or 15.6％ of male or female patients attending STD clinic, respectively. Prevalence of chlamydial EIA－ IgG/MIF－ IgG antibodies was 46.7％ / 11.9％ in pregnant women, 70.2％ /39.9％ in PID patients, 73.5％ /52.4％ and 75.4％ /63.1％ in male and female STD patients, which were statistically higher than those of controls (except pregnant women). Women, rather than men, with genital C. trachomatis were more likely to have serum chlamydial antibodies than those without infection. Eight subjects were measured with acute antibodies to C. trachomatis. Conclusions There is strong relation between cervical C. trachomatis and serum chlamydial antibodies. Serum antibodies to C. trachomatis are of no help for the diagnosis of genital C. trachoamtis infections. Routine screening of C. trachomatis is needed for patients with PID but not for pregnant women.