复方中药对小鼠B-16黑素瘤细胞株黑素合成和酪氨酸酶的激活作用

Effect of Traditional Chinese Medicine on the Tyrosinase Activity and Melanogenesis in B－ 16 Murine Melanoma

目的 研究复方中药对小鼠 B－ 16黑素瘤细胞株细胞增殖、黑素合成的影响，及其对细胞内酪氨酸酶的激活作用。方法 四甲基偶氮唑蓝 (MTT)比色法测定中药对细胞增殖的影响；酶学方法研究中药对酪氨酸酶活性的作用； 470 nm比色标准曲线法测定黑素含量。结果 研究发现复方 3号（补肝肾为主）促进黑素细胞增殖和色素合成的能力高于复方 1号（光敏感性药物）或复方 2号（活血化瘀），而酶激活能力复方 1号较为稳定。结论 在白癜风治疗中补肝肾类中药不可忽视。同时也说明影响黑素合成的因素是多方面的，可能不仅仅与酪氨酸酶活性有关。

Objective To study the effect of traditional Chinese medicine on tyrosinase activity and the melanogenesis of B－ 16 murine melanoma cell. Methods MTT was used to study the proliferation of B－ 16 murine melanoma cell. The tyrosinase activity was measured by enzyme－ kinetic－ method. The melanin was detected by its absorption at 470nm against the standard concentration curve. Results The complex prescription 3(for invigorating liver and kidney) had a strong potential to promote the proliferation and melanogenesis of B－ 16 cell than complex prescription 1 (light sensitive drugs) or complex prescription 2 (for improvement of blood circulation), whereas complex prescription 1 was more stable in promoting tyrosinase activity among these remedies. Conclusion The remedies for invigorating of liver and kidney can not be neglected in vitiligo treatment.