灭蚊真菌Pythium sp.GY1938“菌丝体-1/4KPYG_2凝胶剂”的剂型研制与应用

Study and use of Mycelium-1/4 KPYG_2 Gel containing the mosquito-killing fungus Pythium sp.GY1938

目的探索灭蚊真菌Pythiumsp.GY1938菌株凝胶剂剂型的制备方案。方法根据蚊虫的变态发育特点和Pythiumsp.GY1938菌株的生物学特性,结合保藏、运输和使用的便捷性,以含海藻糖抗逆保护剂的1/2KPYG2定型固体培养基为载体、以菌丝体为活性物质,加工制成"菌丝体-1/4KPYG2凝胶剂"新剂型,在实验室和模拟野外环境下观察其灭蚊效果。结果在实验室和模拟野外环境,每100ml水体使用2块1.51.5×1.0cm3凝胶剂,7d致倦库蚊幼虫致死率均可达到28%。4～8℃条件下凝胶剂品相稳定,储存6个月药效无明显变化。结论 Pythiumsp.GY1938菌株"菌丝体-1/4KPYG2凝胶剂"具有杀灭蚊幼虫作用。该凝胶剂生产工艺简单,独立包装,贮存方便,使用时不需要专用器械,拆开包装袋直接投放或捏碎后投入水体中,不污染环境,具有较好的应用前景。

Objective To explore the best method of preparing a gel containing the mosquito-killing fungus Pythium sp.GY1938.Methods Based on aspects of mosquito development and the biological characteristics of Pythium sp.GY1938,Mycelium-1/4 KPYG2 Gel was prepared using solid medium with 1/2 KPYG2 containing trehalose as a carrier and Pythium sp.GY1938 mycelia as the active ingredient.Storage,transportation,and ease of use were considered.The fungus＇ effectiveness at killing mosquitoes was also determined in experiments and simulations of outdoor conditions.Results In the lab and simulations,2 slabs of gel（1.5×1.5×1.0 cm^3） per 100 ml water killed as many as 28% of mosquitoes in 7 d.The gel remained effective when kept for 6 mo at 4-8 ℃.Conclusion Mycelium-1/4 KPYG2 Gel does not cause contamination and is promising because it is simple to produce and easy to use.