中国小型猪冠状动脉球囊扩张术后的组织学及形态学特征

Morphologic and histological characteristics of coronary artery after balloon overstretch in Chinese mini swine

研究中国小型猪冠状动脉球囊扩张术后的组织学及形态学特征 ,建立中国小型猪冠状动脉内介入术后再狭窄动物模型。方法 :对 16头中国小猪左冠动脉前降支或回旋支行球囊过度扩张术 ,分别在术后 3d和术后 30d处死动物 ,通过HE、马宋三色和维尔霍夫 范吉荪染色切片 ,观察血管最大内膜厚度、新生内膜面积、狭窄程度、血管周长和管腔周长的变化 ;通过免疫组织化学观察α 平滑肌肌动蛋白 (α smoothmuscleactin ,α SMA)阳性细胞分布。结果 :球囊扩张术后 30d组最大内膜厚度、新生内膜面积、血管狭窄程度均明显增加 ,而血管周长和管腔周长均减少 ,其差异均具有显著性意义 ;球囊扩张术后 3d ,血管外膜区可见炎性细胞浸润 ;球囊扩张术后 30d外膜出现大量的纤维素样物质沉积 ,同时血管损伤处外膜出现大量的α SMA阳性细胞。结论 :在球囊扩张术后 30d ,建立了中国小型猪冠状动脉介入治疗后再狭窄动物模型。

Objective:We aim to investigate the morphologic and histologic characteristics of coronary artery after balloon over stretch in Chinese mini swine and to establish restenosis model after intracoronary artery intervention in Chinese mini swine. Methods:Sixteen Chinese mini swine underwent coronary balloon over stretch to LAD and CLX. The animals were sacrificed at three and thirty days following the initial procedure. Cross sections were stained hematoxylin eosin, Masson's trichrome, and Verhoef van Giesen. Maximal intimal thickness, neointima area, percent stenosis, external elastic perimeter and internal elstic perimeter were determined and compared between the two groups. Immunohistochemistry was used to determine the distribution of α smooth muscle actin positive cells. Results:There was a great increase in neointimal area, maximal neointima thickness and percent area stenosis, and there was a great decrease in the external and internal elastic perimeter 30 days after coronary artery balloon injury. Inflammatory cells were observed in adventitia 3 days after the injury. α smooth muscle actin positive cells and fibrosis in adventitia were significantly increased 30 days after the injury. Conclusion:We establish restenosis model after coronary artery intervention in Chinese mini swine.