Wilms肿瘤基因反义寡苷酸对白血病细胞凋亡的影响

Effect of WT1 antisense oligonucleotide on apoptosis of leukemic cells

了解Wilms肿瘤基因 (WT1)反义寡核苷酸 (ASO)对白血病细胞凋亡的作用。方法 :应用WT1ASO以及足叶乙甙 (VP 16 )作用K5 6 2、HL 6 0细胞系 ,然后应用流式细胞仪测定DNA含量以确定白血病细胞的凋亡数。结果 :K5 6 2细胞系经WT1ASO作用 2 4h和 6 0h后细胞凋亡数分别为 14.6 %和 2 6 .8% ;而WT1有义寡核苷酸 (SO)组分别为 3.1% (2 4h)和 3 9% (6 0h) ;加入少量VP 16后凋亡数达到 37 2 % (2 4h)和 6 6 .6 % (6 0h)。HL 6 0细胞经WT1ASO作用 2 4h及 6 0h后细胞凋亡数无明显增高 ,但作用 6 0h后与VP 16联合作用 ,细胞凋亡可达 34.7% ,大于单用VP 16 (13 .7% )及VP 16 +SO (15 .4% )诱导的细胞凋亡数。结论 :WT1ASO可以导致K5 6 2细胞凋亡并可提高白血病细胞对VP 16的敏感性。WT1基因与白血病细胞的凋亡有关 ,其在不同细胞中所发挥的作用不同。

Objective:To elucidate the effect of WT1 antisense oligonuclenotide (WT1 ASO) on apoptosis of leukemia cells and the role of WT1 gene in apoptosis of leukemia cells. Methods:K562, HL 60 cells were treated with WT1 ASO and VP 16. Then apoptosis was quantified by flow cytometric analysis assay. Results:WT1 ASO could induce apoptosis of k562 cells and the level of apoptosis increased markedly when combined with VP 16; WT1 ASO alone couldn't induce apoptosis of HL 60 cells, but it could also increase the level of apoptosis of HL 60 cells when combined with VP 16.Conclusion:WT1 ASO could increase the susceptibility of leukemia cells to VP 16 and WT1 gene may play a role in apoptosis of leukemic cells and the rule in different cells is variable, which is influenced by the type of cell.