MDR1、GST-π、p53、bcl-2在上皮性卵巢癌的表达及意义

The expression of p53, bcl-2,MDR1,GST Pi protein in ovarian epithelial cancer

探讨MDR1、GST π、p5 3、bcl 2与上皮性卵巢癌临床病理学特性的关系。 方法 :采用免疫组化SP法检测 89例不同性质卵巢组织中MDR1、GST π、p5 3、bcl 2的表达情况。结果 :①上皮性卵巢癌 ,交界性肿瘤 ,良性上皮性肿瘤 ,正常卵巢组织中四种指标的表达情况 :MDR1依次为 2 2 / 5 2 (4 2 .3 % )、3/ 9(33 .3 % )、0 / 2 1、0 / 7,GST π依次为 43/ 5 2 (82 .7% )、5 / 9(5 5 .6 % )、1/ 2 1(4 .8% )、0 / 7,p5 3依次为 34/ 5 2 (6 5 .4% )、1/ 9(11.1% )、0 /2 1、0 / 7,bcl 2依次为 31/ 5 2 (5 9.6 % )、5 / 9(5 5 .6 % )、2 / 2 1(9.5 % )、1/ 7(14.3% )。四个指标在上皮性卵巢癌中的表达率显著增高 ;②化疗耐药与MDR1和GST π相关 ;③p5 3表达与肿瘤分期和组织分化程度相关 ;bcl 2表达与一定的组织类型相关 ;④p5 3与bcl 2 ;p5 3与MDR1;bcl 2与GST π间表达具有一定相关性。结论 :MDR1、GST π、p5 3、bcl 2的过度表达在上皮性卵巢癌中常见 ,并与一定的临床病理情况有关 ;MDR1和GST π的检测有助于临床预测化疗效果 ;凋亡调节基因与耐药相关基因的关系需进一步探讨。

Objective:To study the relationship between MDR1, GST Pi, p53 and bcl 2 with the clinical pathological characteristics of oavaian epithelial cancer and evaluate their role in the origin, progression, therapy and prognosis of ovarian epithelial cancer. Methods:The expression of MDR1, GST Pi, p53, bcl 2 protein were detected by immunohistochemical staining in 52 cases of ovarian cancer, 9 cases of borderline malignancy, 21 cases of benign tumor and 7 cases of normal ovarian tissue. Results:1. In normal ovary, benign tumor, borderline malignancy and ovarian cancer, the positive of MDR1 protein (P glycoprotein) was 0, 0, 3/9, 22/52; the positive of GST Pi protein was 0, 1/21, 5/9, 43/52; the positive expression of p53 protein was 0, 0, 1/9, 34/52; and the positive of bcl 2 protein was 1/7, 2/21, 5/9, 31/52 respectively. 2. The expressions of MDR1 and GST Pi protein were closely related to non despondence to chemotherapy. 3. The expression of p53 protein revealed significant relationship to the clinical stage and the histological differentiation; and bcl 2 protein was closely related to histological type. 4. Significant relationship was found between p53 and bcl 2, p53 and MDR1, bcl 2 and GST Pi. Conclusion:Overexpression of MDR1, GST Pi, p53, bcl 2 in ovarian epithelial cancer is very common. Four genes involve in the origin and development by different means and have different relationship with ovarian cancer .Determination of the expression of MDR1 and GST Pi is useful for predicting the sensitivity to chemotherapy. The relationship between apoptosis and drug resistance gene is worth to be further studied.