电针治疗对不同程度绝经后骨质疏松症大鼠一氧化氮、一氧化氮合酶的影响

Effect of electro - acupuncture on the expression of NO and iNOS from the SD rats with varying degrees

目的探讨针刺防治对不同程度骨量丢失的绝经后骨质疏松症大鼠一氧化氮(NO)、一氧化氮合酶(iNOS)的影响。方法选用10～12月龄清洁级雌性SD大鼠分组:假手术大鼠为对照组;去势大鼠按骨量丢失程度分层,按随机数字表法分为模型组、电针组、药物组、电针加药物组,每组10只。假手术组不摘除卵巢,其余4组均摘除双侧卵巢。术后8周,取血液分别用采用硝酸还原酶法测定血清中NO的含量;采用iNOS分型试剂盒测定血清iNOS活性。结果去势SD大鼠血清NO、iNOS的含量有不同程度的降低;电针和药物阿仑磷酸钠能提高血清NO、iNOS的含量且功效相同(P>0.05),电针和药物阿仑磷酸钠共同使用,能明显增强其功效。结论电针可能通过调节不同程度绝经后骨质疏松症SD大鼠体内NO、iNOS含量,进而调节雌激素等间接调节骨的吸收和重建;同时抑制成熟破骨细胞的生物活性并且能诱导破骨细胞前体的凋亡、抑制破骨细胞的形成。

Objective The mechanism of acupuncture on the prevention and therapy of the model rats on varying degrees of osteoporosis is discussed by the gene expression of NO and iNOS. Methods Ten to twelve months old female rats were divided into five groups： sham operation group is for comparison; the SD rats were defined on the degree of the bone loss, and randomized into model group, electro acupuncture group, medicine group, and a electro acupuncture ＆ medicine group. And each group has ten rats. The rats in sham operation group were not ovariectomized, and the others were. After eight weeks, the NO content in blood serum were detected by The nitrate reductase assay. The iNOS＇ activity were detected by iNOS - type kit. Results The content of NO and iNOS in ovariectomized SD rats＇ blood serum was decrease in varying degrees. The electro acupuncture group and the medicine group （alendronate） both increased the content of NO and iNOS （ P 〉 0.05）. And the electro acupuncture ＆ medicine group even has the better effect. Conclusion Electro acupuncture may by regulation postmenopausal osteoporosis SD rats of NO, iNOS content, thereby regulating estrogen indirect regulation of bone resorption and reconstruction; while inhibiting the biological activity of mature osteoclasts and induce osteoclast precursorapoptosis, inhibition of the formation of osteoclasts.