肺泡Ⅱ型上皮细胞染色体脆性位点基因WWOX与特发性肺间质纤维化的关系

Relationship between fragile sites gene of WWOX in type Ⅱ pneumocytes and idiopathic pulmonary fibrosis

目的研究肺间质纤维化模型大鼠肺泡Ⅱ型上皮细胞染色体脆性位点基因WWOX表达的改变。方法 SD雄性大鼠随机分为假手术组、模型组及川芎嗪预防用药组,每组6只,分别于给药7、14、28 d后提取肺泡Ⅱ型上皮细胞体外培养,免疫组织化学法检测WWOX蛋白表达。结果与假手术组比较,注射博莱霉素7 d后模型组大鼠肺泡Ⅱ型上皮细胞染色体脆性位点基因WWOX蛋白表达无显著变化。用药14、28 d后,WWOX蛋白表达则明显下降。而川芎嗪预防用药组WWOX蛋白表达比模型组有显著上调。结论肺间质纤维化过程中可能存在染色体脆性位点基因WWOX的改变,川芎嗪可以在一定程度上抑制这种改变而发挥防治作用。

Objective To investigate changes in expression of WWOX,a fragile sites gene,in type Ⅱ pneumocytes isolated from rats with idiopathic pulmonary fibrosis.Methods SD rats were randomly divided into 3 groups（n=6 in each group）： sham-operated group,model group and tetramethylpyrazine group.After given bleomycin,the rats were harvested at 7、14 and 28 day to isolate the type Ⅱ pneumocytes and then culture the cells in vitro.Then,we detected the protein expression of WWOX in pneumocytes with immunocytochemistry.Results Compared with the sham-operated group,no significant differences in WWOX protein expression were found in rat type Ⅱ pneumocytes of the model group administrated with bleomycin for 7 days.After 14 and 28 days of administration,the WWOX protein expression was reduced greatly in the model group.Nevertheless,tetramethylpyrazine enhanced significantly WWOX protein expression compared with the model group.Conclusion In this study,we demonstrate that the expression of fragile sites gene of WWOX is altered during idiopathic pulmonary fibrosis,while tetramethylpyrazine suppresses the alteration to some extent,suggesting the medication could play a protective role for idiopathic pulmonary fibrosis.