肝靶向抗癌药NGA—DHAQ中DHAQ含量的测定方法

Quantitative determintion of DHAQ in hapatic targeting antineoplastic drug NGA—DHAQ

目的：测定肝靶向抗癌药NGA—DHAQ中DHAQ的含量。方法：通过均匀设计和正交设计得到肝靶向化学偶联物NGA—DHAQ发生水解而游离出DHAQ的最佳条件：0.5mlNGA—DHAQ液透析中，加入430μl水和70μl浓HCl，水浴90℃恒温40分钟。并在此条件下初步考察了DHAQ的稳定性。结果：建立了NGA—DHAQ中DHAQ含量的测定方法。结论：说明此方法可行。

Objective: Quantitative determination of DHAQ in hapatic target ing antineoplastic drug NGA—DHAQ. Methods: An optimal hydrolytic condition which was used for determination of DHAQ in hepatic targeting drug NGA— DHAQ was developed by the Orthogonal and uniform designed experiment.The result was 430μl H\-20 and 70μl HCl mixed 0.5ml NGA—DHAQ dialysis liquid in 90℃ for 40 min. Moreover, the stability of DHAQ in above condition was also inspected. Results :A quantitative determination of DHAQ in hapatic targeting antineoplastic drug NGA—DHAQ was established. Conclusions: The meth od proved to feasible.