Dazl基因mRNA表达水平与犏牛雄性不育关系的研究

The Study on the Relationship between the Expression Level of Dazl gene mRNA and the Infertility of Cattle-yak

本文旨在探讨Dazl基因与犏牛雄性不育的关系。采用实时荧光定量PCR技术检测黄牛、牦牛和犏牛睾丸组织中Dazl基因mRNA表达并进行分析。结果表明:三种牛中Dazl基因在睾丸组织中特异表达,且犏牛睾丸组织中表达量最低,其中黄牛与牦牛、黄牛与犏牛差异显著(P<0.05),犏牛与牦牛差异不显著(P>0.05)。结果提示,Dazl基因在三种牛睾丸组织特异表达,说明Dazl基因在细胞周期运行中发挥作用,Dazl基因在犏牛睾丸组织中低表达与犏牛雄性不育相关。

This study was conducted to study the relationship between the infertility of cattle - yak and the expression level of Dazl gene mRNA. The expression profiles were obtained by RT -PCR. Dazl gene was specifically expressed in cattle, yak and cattle - yak testis tissues, which confirmed it plays important role in the progression of cell cycle. Real- time quantitative PCR analysis indicated that the expression levels of Dazl gene in cattle and yak testis were higher than its in cattle - yak. There were significant difference between cattle and cattle yak, yak respectively （ P 〈 0.05 ）. Therefore, low expression level of Dazl gene may have a relationship with the infertility of cattle - yak.