腹腔感染细菌学的调查研究

Microbiology of Intra-Abdominal Infectoion

目的 :对目前腹腔感染的致病菌进行流行病学调查。 方法 :185例因腹腔感染入院患者 ,分离标本包括胆汁、阑尾腔内容物、腹腔液、切口分泌物等。采用VITEK自动分析鉴定仪进行需氧菌分离鉴定 ,采用VITEK -Amsani软件、VITEK厌氧菌鉴定卡进行厌氧菌分离鉴定。 结果 :(1)在 185例标本中 ,细菌阳性检出率为 74 6 % ,其中需氧菌检出率占 75 6 2 % ,厌氧菌占 2 4 38% ;(2 )需氧菌中以大肠杆菌为主 ,厌氧菌中以类杆菌为主 ;(3)上腹部以需氧菌为主 ,下腹部以需氧菌与厌氧菌混合感染为主。 结论 :腹腔感染主要的致病菌仍然是大肠杆菌和类杆菌 ,肺炎克雷白氏杆菌有上升趋势。

Objective:To focus on epidemiological investigation of intra abdominal infection on microbiology.Methods:185 samples of in patients suffering from intra abdominal infection were obtained for culture and identification,including bile,appendix cavity contents,peritoneal fluid,secretion from incisional wounds.Aerobic bacterium were indentified by VITEK autobiochemistry analysis equipment.Anaerobic bacterium were identified by VITEK Ams ani system.Results:(1)In all samples,positive rate of isolation was 74.6%,among them aerobe was 75.62%,and anaerobe,24.38%.(2)E Coli was the dominant species of aerobic bacterium,whereas Bacteriobes spp.the dominant species of abaerobic bacterium.(3)There were some differences in species at different anatomy location.Conclusion:E Coli and Bacteriobes spp.remains to be the dominant pathogenic bacterium,with Klebsiella pneumonia as the less dominant bacterium in current intra abdominal infection.