脾切除对肝纤维化大鼠肝脏结缔组织生长因子的表达和血清水平的影响

The effects of splenectomy on the expression of CTGF in the liver and the serum level of CTGF of the rats with hepatic fibrosis

目的研究脾切除对肝纤维化大鼠肝脏结缔组织生长因子(CTGF)的表达和血清CTGF水平的影响,探讨脾切除在肝纤维化治疗中的作用。方法采用CCL4诱导法建立肝纤维化模型;所有老鼠随机分为A、B、C组,并分别于建模0时、第5周、第10周切除脾脏;3组均在切脾前随机选择5只老鼠处死,剩余老鼠在建模第14周时全部处死,取其肝脏和血液标本,ELISA方法检测血清中CTGF含量,肝组织分别行HE染色和免疫组化染色。结果肝损伤不同时期大鼠肝脏及血清CTGF测定值表明,大鼠肝脏及血清CTGF含量与纤维化程度呈正相关;不同时期切脾鼠肝脏及血清CTGF值与切脾早晚呈相关关系,切脾越早,CTGF值越低。

Objective To discuss the effects of splenectomy on the expression of CTGF in the liver and the serum level of CTGF of the rats with hepatic fibrosis,and explore the effect of splenectomy on the treatment for hepatic fibrosis.Methods Cirrhosis was induced in rats by hypodermic injection CCL4.All the models were divided into three groups randomly at the beginning.The splenectomy were performed in the three groups at different phases：before hypodermic injection CCL4（A group）,fifth week after hypodermic injection CCL4 （B group）,tenth week hypodermic injection CCL4（C group）.Then took samples of the livers and the serum of the rats in different phases（before splenectomy and the fourteenth week）.The degrees of hepatic fibrosis were detected by HE staining and the expressions of CTGF in the liver were detected by immunohistochemistry technique.The serum levels of CTGF were analyzed by ELISA technique.Results The levels of CTGF in the liver and the serum of rats were positively correlated with the degree of fibrosis,the earlier was splenectomy,the lower was the levels of CTGF in the liver and serum.Conclusion Splenectomy could delay the progression of liver fibrosis.