肺表面活性物质相关蛋白A基因多态性与儿童支原体肺炎易感性的研究

Surfactant protein A gene polymorphisms in child mycoplasma pneumonia

目的探讨肺泡表面活性物质蛋白A(SP-A)基因多态性与儿童支原体肺炎发病风险的相关性。方法应用基因测序技术检测SP-A基因在100例支原体肺炎患儿及100例非支原体肺炎患儿中基因分布的频率;观察两组间基因型频率和等位基因频率的差异。结果病例组患儿100例的+186位点AA、AG、GG3种基因型的分布频率分别为73%、26%、1%,而对照组为61%、30%、9%,两组比较差异有统计学意义(P<0.05);+655位点CC、CT、TT基因型的分布频率分别为76%、23%、1%,对照组为52%、36%、12%,两组比较差异也有统计学意义(P<0.05);+667位点AA、AG、GG3种基因型的分布频率分别为28%、47%、25%,而对照组这3个基因基因型的分布频率分别为35%、51%、14%,两组比较差异无统计学意义(P>0.05)。结论 SP-A基因+186位点及+655位点基因多态性可能与儿童支原体肺炎易感性有关,但该位点的突变如何影响SP-A的表达和活性,还有待进一步的研究。

Objective To investigate the association between the surfactant protein A gene polymorphisms and child mycoplasma pneumonia.Methods Using gene sequencing technologies to test the surfactant protein A gene in 100 children with mycoplasma pneumonia（the infected group） and 100 cases without mycoplasma infections（the uninfected group） and to contrast the differences.Results In infected group the fequencies of ＋186 locus genotypes AA,AG and GG were 73%,26% and 1%,in the uninfected group these were 61%,30% and 9%.And the ＋655 locus genotypes CC,CT and TT were 76%,23% and 1%,in the uninfected group these were 52%,36% and 12%.There were statistically significant between two groups（P0.05）.The frequency of polymorphic genotypes at ＋667 locus showd no statistical significance between two groups（P0.05）.Conclusion SP-A gene polymorphisms at ＋186 locus and ＋655 locus may be connected with child mycoplasma pneumonia.