体外培养血管平滑肌细胞与内皮细胞增殖的影响

MODULATIONS OF PROLIFERATION BETWEEN VASCULAR ENDOTHELIAL CELL AND VASCULAR SMOOTH MUSCLE CELL IN VITRO

研究在体外培养条件下兔血管内皮细胞条件培养基 (ECCM)、内皮素 - 1(ET- 1)、内皮素转化酶抑制剂(Endothelial- coverting enzyme inhibitor) Phosphoram idon对血管平滑肌细胞 (SMC)增殖的影响 ,同时研究了血管平滑肌细胞条件培养基 (SMCCM)对血管内皮细胞 (EC)增殖方面的影响。方法 :EC和 SMC均来源于兔主动脉 ,在获得了 EC和SMC的条件培养基 (CM)后 ,分别用两者以及 ET- 1进行实验 ,细胞的增殖率通过 3H掺入法进行测定。结果 :ECCM和 ET-1可明显促进 SMC的增殖 ,并呈现剂量依赖性。其最大效应分别为 190 %± 11% (10 0 % ECCM)和 16 6 %± 9% (10 0 pg/m lET- 1)。而 Phosphoramidon存在条件下的 ECCM使其分裂作用降低 33%± 2 %。SMCCM抑制 EC的增殖 ,这种抑制作用并非剂量依赖性 ,其最大的抑制效应为基本水平的 78%± 3%。结论 :ECCM和 ET- 1可促进 SMC的增殖。Phosphoramidon可明显地抑制 ECCM对 SMC的增殖作用。同时 SMCCM对 EC的增殖起抑制作用。

Aim:To study the effects of vascular endothelial cell conditioned medium(ECCM), endothelin-1 (ET-1), Endothelin-converting enzyme inhibitor Phosphoramidon on the proliferation of vascular smooth muscle cell (SMC) and vascular smooth muscle cell conditioned medium(SMCCM) on the proliferation of vascular endothelial cell(EC)in vitro.Methods:EC and SMC were isolated from rabbit aortas and cultured.The conditioned medium(CM) of EC and SMC were harvested.ECCM,ECCM conditioned in the presence of Phosphoramidon,SMCCM and ET-1 were added individually to the cultures.Proliferation rate of the cells was measured with 3 H-thymidine incorporation on 3 days. Result:ECCM and ET-1 significantly increased the proliferation of SMC in a dose-dependent manner with a maximal effect of 199%±11%(100% ECCM) and 166%±9%(100pg/ml ET-1)respectively.ECCM conditioned in the presence of Phosphoramidon reduced the mitogenic effect by 33%±2%.SMC conditioned medium elicited a dose dependent decrease of cultured EC proliferation with a maximal effect of 78%±3% over basal level. Conclusion:ECCM and ET-1 had a mitogenic effect on SMC.Phosphoramidon could significantly reduce the mitogenic effect of ECCM.SMCCM had an effective inhibition on EC proliferation.