CD-_(10)的B-ALL和NHL以及CLL免疫表型检测结果分析

Analysis of the immunophenotypes of CD- _(10) B-ALL, NHL and CLL

目的 比较急性B淋巴细胞性白血病 (B ALL) ,非霍奇金淋巴瘤 (NHL)和慢性淋巴细胞性白血病(CLL)免疫表型表达的区别。方法 采用ABC AP法结合多种B淋巴细胞相关单克隆抗体 ,对 78例初发B ALL(均为CD-10 的病例 )、2 1例初发NHL和 2 5例初发CLL患者进行免疫表型检测。结果 CD-10 的B ALL患者的白血病细胞以CD19和HLA DR呈高阳性率表达 ;NHL患者的肿瘤细胞以CD2 0 和HLA DR呈高阳性率表达 ;CLL患者的白血病细胞除CD+ 19、CD+ 2 0 、CD+ 2 2 和HLA DR+ 等B淋巴细胞相关抗体表达外 ,主要还对CD5有很高的阳性率表达。结论 利用细胞免疫表型检测 ,对于临床上症状相似而仅以细胞形态学检查无法明确诊断的急性淋巴细胞性白血病、恶性淋巴瘤或慢性淋巴细胞性白血病 ,可能起到区别细胞类型 。

Objective To compare the differences in expressing the immunophenotype of B acute lymphocytic leukemia with CD - 10 (CD - 10 B ALL), non Hodgkin's lymphoma (NHL) and chronic lymphocytic leukemia (CLL). Methods The immunophenotypes of 78 CD - 10 B ALL cases, 21 NHL and 25 CLL cases were determined by a panel of B lymphocytic monoclonal antibodies with ABC AP method. Results CD + 19 cells in CD - 10 B ALL were higher than CD + 20 cells. On the contrary, CD + 20 cells in NHL were higher than CD + 19 cells. In CLL, the expression of CD 5 was higher than that of CD 19 , CD 20 , CD 22 and HLA DR. Conclusion The immunotyping technique can be used to diagnose acute lymphocytic leukemias, malignant lymphomas or chronic lymphocytic leukemias whose clinical features are very much alike and differentiation being difficult by morphologic analysis only. (Shanghai Med J, 2000,23∶354 356)