输卵管组织中内皮素-1基因表达的实验研究

Experimental study of endothelin-1 gene expression in uterine tube tissue

目的 :为了探明内皮素 (ET)在输卵管组织中的分布及来源 ,以便进一步探讨内皮素 - 1(ET - 1)与输卵管功能调节之间的关系。方法 :用SABC免疫组织化学法和地高辛精标记的ET - 1cRNA探针核酸原位杂交组织化学法 ,观察了兔输卵管组织ET - 1的分布和ET - 1mRNA的表达。结果 :输卵管粘膜上皮细胞内可见被染成红色的ET - 1颗粒 ,主要分布在细胞核的上方 ,靠近游离面 ,在肌层可见少量的ET - 1颗粒。输卵管粘膜上皮细胞内可见呈深兰色的ET - 1mRNA阳性杂交信号 ,阳性杂交信号强且密集 ,主要分布在细胞核的上方 ,靠近游离面 ,在肌层未见ET - 1mRNA阳性杂交信号。结论 :表明兔输卵管粘膜上皮细胞自分泌ET - 1。

AIM: The distribution of endothelin-1(ET-1) in uterine tube and its source were investigated in order to find out relation between endothelin-1 and function of uterine tube.METHODS: The distribution of endothelin-1 and the expression of ET-1 mRNA in the rabbits uterine tubes were studied using SABC immunohistochemistry and in situ hybridization histochemistry with digoxigenin-labelled rat ET-1 cRNA probe. RESULTS: ET-1 granules with red color were seen in the uterine tube epithelial cells and they were located mainly above the cell nucleus and under the cell surface membrane. Less ET-1 granules were seen in the myometrium of uterine tube. ET-1 mRNA positive hybridization signals with deep blue were distributed in the uterine tube epithelial cells. These signals were strong and dense. They were distributed mainly above the nucleus and near the cell surface membrane. ET-1 mRNA positive hybridization signal was not seen in the myometrium of uterine tube.CONCLUSION: Our results indicate that epithelial cells of rabbit's uterine tube epithelial cells secrete ET-1.