瓜蒌注射液对兔血管平滑肌细胞增殖细胞核抗原表达的影响

Effect of trichosanthes injection on expression of proliferating cell nuclear antigen of vascular smooth muscle cell in rabbit

目的 :观察瓜蒌注射液对血管平滑肌细胞 (SMC)增殖细胞核抗原 (PCNA)表达的影响。方法 :用免疫组织化学LSAB法检测培养的兔主动脉SMC中PCNA的表达 ,液闪法测定SMC氚 -胸腺嘧啶核苷 ([3H]-TdR)掺入量 ,同时测定培养液中超氧化物歧化酶 (SOD)、脂质过氧化物 (LPO)、前列环素 (PGI2 )及环磷酸腺苷(cAMP)的含量。结果 :瓜蒌注射液能增加SOD活性 ,降低LPO和升高PGI2 、cAMP水平 ,抑制SMC的 [3H]-TdR掺入量和PCNA的表达 (P均 <0 0 5 ,0 0 1)。结论 :瓜蒌有抑制SMC增殖的作用。

AIM: To observe the effect of thichosanthes injection on the expression of proliferating cell nuclear antigen (PCNA) of vascular smooth muscle cell (SMC). METHODS: The expression of PCNA of cultured rabbit aortic SMC was examined with LSAB immunohistochemical technique, and [ 3H]-thymidine([ 3H]-TdR) incorporation data of SMC and the contents of superoxide dismutase (SOD), lipid peroxide (LPO), prostacyclin (PGI 2) and cyclic AMP (cAMP) in medium were simultaneously determined. RESULTS: Thichosanthes injection has an effects of increasing SOD activity, decreasing LPO, elevating PGI 2 and cAMP, reducing [ 3H]-TdR incorporation and expression of PCNA (all P< 0 05, P <0 01). CONCLUSION: Thichosanthes could inhibit SMC proliferation.