阿霉素对大鼠心肌损伤的实验研究

Experimental Study of Doxorubicin on Myocardial Injury

目的观察经阿霉素(ADR)作用的实验大鼠心肌组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、黄嘌呤氧化酶(XOD)含量的变化。方法采用36只Wistar雌性大鼠,分生理盐水(NS)组和ADR组,ADR组尾静脉缓慢注射ADR(3mg/kg),每周一次;NS对照组尾静脉及腹腔注射等剂量NS。分别用药三周和六周后处死,观察各组大鼠心肌组织中MDA、SOD、GSH及XOD含量的变化。结果 ADR组MDA与XOD含量均升高,SOD与GSH含量均下降。结论过氧化反应增强、自由基生成酶活性升高及清除酶或清除剂活性降低可能参与了ADR心脏损伤的发生。

Objective To observe the changes of the contents of MDA、SOD、XOD and GSH in cardiac homogenate。Methods Tirty-six healthy female Wistar-rats were adopted.ADR was administered slowly by caudal vein injection（3mg/kg body weight） once a week in ADR group.Control rats received equivalence saline by caudal vein injection.These rats were sacrificed separately after three weeks and six weeks.We observed and compared the changes of the contents of MDA,SOD,GSH and XOD in cardiac homogenate.Results Compared with the NS group,the increased contents of MDA and XOD in cardiac homogenate were found in ADR group.Conclusion Peroxidation and the increased activity of the enzymes which produce the free radicals and the decreased activity of the enzymes which eliminate the free radicals involved the occurrence of the adriamycin-induced cardiotoxic.