丙烯酰胺单体对人肝细胞作用的实验研究

Study on anti-proliferation effect of acrylamide on human hepatocyte in vitro

目的探讨丙烯酰胺单体(acrylamide,AA)对体外培养人肝细胞株(L-02)的生长抑制作用。方法 MTT比色法测定不同时间段、不同浓度AA对L-02细胞的生长抑制率,并设对照组,绘制生长抑制曲线。结果经不同浓度(0.625%、1.25%、2.5%、5%、10%、20%)的AA作用24 h、48 h、72 h后,与对照组比较,L-02生长抑制率差异均有统计学意义(P<0.05)。AA对L-02细胞均有明显毒性,表现为细胞增殖抑制作用。结论 AA对体外培养的L-02细胞具有增殖抑制作用,且呈时间剂量依赖性。

Objective To explore the growth-inhibition effect of acrylamide(AA) on human hepatocyte L-02 in vitro.Method Method The growth-inhibition rate of L-02 of different concentration of AA in different time section were tested by MTT method,with L-02 cultured with medium free of AA as control group.Anti-proliferation curves were drawn.Result The anti-proliferation rate of L-02 of different concentration of AA(0.625,1.25,2.5,5,10,20%) in different time section(24h,48h,72h) was different to that of the control(P<0.05).AA was showed with apparent cytotoxicity of cytostasis.Conclusion AA has a function of anti-proliferation on L-02 in vitro,and it increases with both time and dose.