普洱茶不同溶剂提取组分降脂减肥作用的比较研究

Comparative Study on Lipid-lowering and Anti-obesity Effect of Different Pu-erh Tea Fractions

采用丙酮、水、氯仿、乙酸乙酯和正丁醇连续萃取普洱茶,旋转蒸发后冷冻干燥得到氯仿层、乙酸乙酯层、正丁醇层以及剩余水层4个主要分离组分,利用不同的组分灌胃经过肥胖造模处理的ICR小鼠,与普洱茶总水提取物和减肥药左旋肉碱进行比较,研究普洱茶不同组分对ICR肥胖小鼠的降脂减肥作用。实验结果表明,普洱茶总水提取物、乙酸乙酯萃取组分、剩余水层组分均对肥胖ICR小鼠具有很好的降脂减肥作用。其中乙酸乙酯层和剩余水层抑制脂肪累积(抑制率分别是24.7%和22.5%)的效果略差于减肥药物左旋肉碱(抑制率38.8%),但在改善血清指标尤其是提高HDL-C水平方面(提高率为61.7%和73.4%)显著优于左旋肉碱(9.4%),肝脏指标检测也得到了类似的结论,揭示普洱茶降脂减肥作用的主要成分可能存在于乙酸乙酯层和(或)剩余水层中。

In this study,Pu-erh tea was extracted with acetone,water,chloroform,ethyl acetate,and n-butanol.then,the extraction was concentrated with rotary evaporation and dried with lyophilizer into chloroform layer,ethyl acetate layer,n-butanol layer and surplus water layer extracts,which were used to administer ICR obese mouse for analyzing the lipid-lowering and anti-obesity effects.The experimental results demonstrated that the pu-erh tea total extract,ethyl acetate extract and surplus water extract are all effective on lipid-lowering and anti-obesity of ICR obese mouse,and the effects of ethyl acetate extract and surplus water extract on inhibiting fat accumulation（inhibition ratio was 24.7% and 22.5%）was slightly less than those in the L-carnitine（38.8%）,but the effect on improving HDL-C level（61.7% and 73.4%） significantly superior to that in the L-carnitine（9.4%）,the similar results were obtained in the mice liver,which suggest that the key lipid-lowering and anti-obesity function components were existed in the ethyl acetate layer and（or） surplus water layer extracts of pu-erh tea.