菌根化马尾松植株抗旱性研究

Drought Resistance of Pinus massoniana Seedlings Inoculated with Ectomycorrhizal Fungi

通过对干旱胁迫下菌根化马尾松松针中细胞膜透性及保护酶的活性变化进行研究,分析了细胞膜保护酶在外生菌根提高马尾松抗旱能力过程中的作用,结果表明,接种劣味乳菇的菌根化马尾松植株的抗旱能力较强,其次是接种紫金蜡蘑的菌根化马尾松植株,CK马尾松植株抗旱能力较差;劣味乳菇菌根化马尾松植株MDA含量和SOD活性的提高幅度均较小,而POD活性和细胞膜透性变化幅度较高,且与马尾松植株的抗旱能力呈正相关。表明细胞膜保护酶的调节作用在外生菌根提高马尾松植株抗旱能力过程中发挥了一定作用。

In order to explore the role of protective enzymes in raising the drought resistance of Pinus massoniana seedlings inoculated with ectomycorrhizal fungi, two mycorrhizal fungi were tested, and the activities of protective enzymes in the needles of the inoculated seedlings were determined under drought stress. The results indicated that the seedlings inoculated with Lactarius insulsus had the highest drought resistance, next came the seedlings inocula- ted with Laccaria aemthystea, and the seedlings without mycorrhizal fungi inoculation, namely the control of the ex- periment had the lowest drought resistance. It was found that inoculating the seedlings with L. insulsus, the content of MDA and the activity of SOD increased slightly, whereas the activity of POD and cell membrane permeability changed significantly, and positively correlated with the drought resistance of the seedlings. It was concluded that the cell membrane protective enzyme playing an important role in drought resistance improvement in Pinus massoni- ana seedlings inoculated with ectomycorrhizal fungi.