甜椒叶绿体小分子量热激蛋白(CaHSP26)对大肠杆菌抗氧化能力的影响

Heterologous Expression of CaHSP26 Gene in Escherichia coli Enhances Its Viability Under Oxidative Stress

旨在探讨大肠杆菌中CaHSP26基因的异源表达对原核生物抗氧化能力的影响。通过构建CaHSP26基因原核表达载体pET30-CaHSP26,经不同浓度过氧化氢处理后,分析大肠杆菌菌种存活率及抗氧化能力。结果表明,与转空载体pET-30a(+)的对照大肠杆菌菌株P30相比,过量表达CaHSP26提高了转基因的大肠杆菌菌株P750过氧化氢胁迫下的存活率,降低了MDA的产生,CaHSP26的表达可以提高原核生物抗氧化能力。试验初步证实超表达CaHSP26的转基因烟草生长势明显高于对照,为今后研究植物中CaHSP26抗氧化能力的作用机理提供依据。

The research is focused on the oxidative stress tolerance of CaHSP26 in Escherichia coli.In the study,CaHSP26 was constructed in the prokaryotic expression vector pET30-CaHSP26,and the expression was induced by IPTG.The antioxidant capacity and cell survivalility induced by H2O2 were detected to study the biological functions of CaHSP26 in prokaryote.The results proved that heterologous expression of CaHSP26 in E.coli highly increased the antioxidant capacity of E.coli and reduced the MDA production compared to the empty vector pET-30a（＋）.We thus suggested that CaHSP26 enhanced the oxidative stress tolerance of prokaryote.