靶向乙酰肝素酶siRNA对大肠癌细胞恶性生物学行为的影响

Effects of targeted heparanase siRNA on malignant biological behaviors of human colon carcinoma cells

目的研究化学合成的siRNA干扰乙酰肝素酶对大肠SW620癌细胞生物学行为及其可能的机制。方法实验组选取对数期生长的SW620癌细胞转染靶向乙酰肝素酶siRNA,对照组用非特异性的siRNA处理对数期生长的SW620癌细胞。应用RT-PCR检测乙酰肝素酶基因表达,并利用免疫组化检测乙酰肝素酶蛋白表达情况,侵袭实验检测细胞侵袭力。结果实验组的对数期生长的SW620癌细胞的乙酰肝素酶基因和蛋白含量显著低于对照组,而实验组细胞的侵袭能力也显著低于对照组。对照组的侵袭细胞数显著多于实验组。结论化学合成的siRNA能够降低大肠癌细胞恶性生物学行为,可作为一种新的基因治疗方法用于大肠癌的临床治疗。

Objective To study the effects of targeted heparanase siRNA on malignant bio- logical behaviors of human colon carcinoma cells and its possible mechanisms. Methods SW620 cancer cells with log growth phase in the experiment group were selected and transfected with targeting heparanase siRNA, and nonspecific siRNA was transfected in the control group. The gene expression of heparanase was detected by application of RT-PCR, and the protein expression was detected by the use of immunohistochemistry. The cell invasion was detected by invasion assay. Re- suits The heparanase gene and protein content of $W620 cancer cells with logarithmic phase growth in the experiment group were lower than those in the control group, while the cell invasion ability in the experiment group was significantly lower than that in the control group. The number of invasive cells in the control group was significantly higher than that in the experiment group. Con- elusion Targeted heparanase siRNA chemical synthesized can reduce malignant biological behaviors of human colon carcinoma cells, which can be used as a new method of gene therapy for clinical treatment of colorectal cancer.