非小细胞肺癌患者呼出气冷凝液VEGF检测的临床意义

Clinical significance of detecting VEGF in exhaled breath condensate of patients with lung cancer

目的测定非小细胞肺癌患者(NSCLC)呼出气冷凝液(EBC)中血管内皮生长因子(VEGF)并研究其临床意义。方法收集40例经病理确诊的NSCLC患者、20例稳定期的慢性阻塞性肺疾病(COPD)患者和20例健康体检者的EBC和血清标本,采用酶联免疫吸附法(ELISA)分别检测EBC和血清中VEGF水平。结果①NSCLC组EBC和血清中VEGF水平[(11.7±6.1)pg/mL、(310.5±23.9)pg/mL]显著高于COPD组[(6.3±4.9)pg/mL、(217.1±14.7)pg/mL]及正常对照组[(3.6±1.2)pg/mL、(162.5±16.5)pg/mL]。②EBC和血清中VEGF水平与NSCLC患者的TNM分期及淋巴结转移呈正相关,与肺癌病理类型及患者性别、年龄、吸烟史无密切关系。结论检测EBC中VEGF有助于肺癌的早期诊断、病情进展及预后判断。

Objective To study the clinical significance of measuring VEGF in exhaled breath condensate（EBC） of patients with non-small cell lung cancer（NSCLC）. Methods Forty patients with confirmed NSCLC by pathology were enrolled in the study. Among them, 20 patients had stable chronic obstructive pulmonary disease （COPD） and the rest 20 were healthy subjects. Serum samples were also collected from the three groups. VEGF levels were measured in EBC and serum of all the subjects under study were applied with enzyme linked immunosorbent assay （ELISA） method. Results（1） VEGF levels in EBC and serum were higher in NSCLC group[ （11. 7±6.1） pg/mL, （310.5 ± 23.9） pg/mL] than those in COPD patients[（6.3 ± 4.9） pg/mL, （217.1 ± 14.7） pg/mL] and healthy controls[ （3.6 ±1.2） pg/mL, （162.5 ± 16.5） pg/mL] ; （2） The VEGF levels in EBC and serum of NSCLC group had a positive correlation with the tumor stages and lymph node metastasis, but were not related to the tumor type, gender, age and smoking history. Conclusion The detection of VEGF levels in EBC is beneficial to the early diagnosis, progress and prognosis of lung cancer.