肝细胞癌血窦CD_(34)表达的意义

Expression of CD_(34) in Sinusoids of Hepatocellular Carcinoma and Liver Cirrhosis

目的比较肝细胞癌、肝硬化和结节状增生中血管内皮细胞标记物CD34 、UEA -Ⅰ和Ⅷ因子的表达及意义。 方法对 36例手术切除肝细胞癌 ,其中 19例癌旁肝硬化、4例结节状增生及 2例正常肝组织进行免疫组织化学标记。 结果 1.CD34 在所有肝细胞癌 (HCC)血窦中均有较强阳性的表达 ,肝硬化 ,结节状增生及正常肝组织血窦中仅 3例有弱阳性的CD34 表达 ;2 .UEA -Ⅰ在HCC、肝硬化及结节状增生中均有较强阳性的血窦表达。CD34 在HCC血窦中表达与肝硬化、结节状增生相比显著增强 (P <0 .0 1) ;UEA -Ⅰ在HCC和肝硬化、结节状增生血窦表达无显著差异 (P >0 .0 5 )。 结论HCC与肝硬化、结节状增生血窦CD34 表达的差异可能与肿瘤的血管形成有关 ,而非简单的毛细血管化 ;CD34

Objective To investigate the expression of CD 34 with other endothelial markers in sinusoids of hepatocellular carcinoma (HCC), livercirrhosis and focal nodular hyperplasia. Methods Immunohistochemistry was used to detect CD 34 , UEA-Ⅰ and Factor Ⅷ to find out and differences in 36 cases of HCC, 4 cases of focal nodular hyperplasia and 2 cases of normal liver tissue. Results CD 34 , UEA-Ⅰ was stained in a large numbers of sinusoids in virtually all of the HCC investigated. It was also stained in a great number of sinusoids in cirrhotic liver and focal nodular hyperplasia. There was signifigant difference at the levels of CD 34 expression of sinusoids between HCC and liver cirrhosis (P<0.01). There was no signifigant difference at the levels of UEA-Ⅰ expression of sinusoids between HCC and liver cirrhosis (P>0.05). Conclusion It suggests that the increase in immunoreactivity of sinusoids in HCC with anti-CD 34 , unlike that with UEA-Ⅰ and Factor Ⅷ, is not an expression of capillarization, but rather of angiogenesis. The marked expression of CD 34 by HCC sinusoids could be of practical value for distinguishing between differentiated HCC and severe dysplasia.