摘要
目的采用实时定量PCR方法检测食管癌样本中Smad4基因mRNA的表达量,分析其与临床资料之间的关系。方法设计Smad4基因特异性探针和引物,建立Smad4基因实时定量检测方法;检测40例食管癌患者(男28例,女12例,38~82岁,中位年龄60岁)肿瘤切除石蜡标本中Smad4基因mRNA的模板拷贝数,以beta-actin为内参得出校正过的基因表达相对值。统计学分析这些值与临床病理及预后的关系。结果 Smad4的mRNA表达水平与性别、年龄、淋巴结转移、病理亚型无关(P>0.05);与细胞分化和病理分期有关(P<0.05)。Smad4低表达的患者预后较高表达者差(P=0.009)。结论食管癌中Smad4mRNA表达的可作为预测淋巴结转移、评价预后的一个重要指标。Smad4可能是食管癌变过程中的重要分子。
Objective To detect the expression level of smacl4 mttP^A m me sampte o~ e- sophageal carcinoma by using real-time quantitative PCR method analyze its relationship with clinical materials. Methods To design specific probe and primer for gene Smad4 and to establish real-time quantitative PCR method for Smad4 as well. Tumor paraffin specimens of 40 esophageal carcinoma pa- tients (2g males and 12 females, age from 3g to g2 years old, median 60 years old) were analyzed in index of template copy number, and the relative value of gene expression was counted by beta-aetin regulation. Relationship between these values and elinicopathology and prognosis were analyzed statis- tically. Results The expression level of smad4 mRNA was positively correlated to pathologic stage and cell differentiation (P 〈 0.05), but not correlated to age, gender, lymphatic metastasis and pathologi- cal subtypes (P 〉 0.05 ). Conclusion Smad4 mRNA in esophageal carcinoma may be an important indicator in predicting lymphatic metastasis and evaluating the prognosis.
出处
《实用临床医药杂志》
CAS
2012年第23期9-11,共3页
Journal of Clinical Medicine in Practice
关键词
食管肿瘤
实时定量PCR
SMAD4
临床病理参数
预后
esophageal carcinoma
real-time quantitative PCR
Smad4
clinieopathological features
prognosis