胰腺癌错配修复基因的表达

The expression of mismatch repair gene in pancreatic carcinoma

目的探讨胰腺癌错配修复基因hMLH1的表达与临床病理特征的关系。方法分别取60例胰腺癌组织及60例正常胰腺组织标本，采用免疫组化SP法检测hMLH1在胰腺癌与正常胰腺组织的表达并比较其差异。结果胰腺癌hMLH1的强表达为0％，弱表达为31．7％（19／60），缺失表达为68．3％（41／60）；正常胰腺组织hMLH1强表达为83．33％（50／60），弱表达为16．67％（10／60），缺失表达为0％，2组比较差异有统计学意义（P〈0．001）。胰腺癌hMLH1蛋白表达在患者年龄、胰腺癌的位置、病理类型等方面差异无统计学意义（P〉0．05），在淋巴结转移（x2=8．579，P=0．004）、临床分期（X2=9．586，P=0．002）、病理分化程度（x2=20．372，P=0．001）方面差异有统计学意义。结论hMLHl基因缺失表达与胰腺癌发生、分化程度、病情进展有关。

Objective To investigate the role of mismatch repair gene hMLH1 in pancreatic carcinoma and its elinicopathological significance. Methods hMLH1 was extracted from 60 cases of pancreatic carcinoma tissues and 60 cases of normol pancreatic tissues, hMLH1 expression in pancreatic carcinoma and normal tissues was detected by SP immunohistochemical staining. Results The strong, weak and loss expression of hMLH1 in pancreatic carcinoma tissues and normal pancreatic tissues was 0 vs 83.33% （ 50/60 ）, 31.7% （ 19/60 ） vs 16. 67% （10/60）, and 68.3% （41/60）vs 0 respectively. The protein expression of hMLH1 was not related to patient＇s age, tumor location, or pathological types （P 〉 0. 05）, but it was related to lymph node metastasis （X2 = 8. 579, P = 0.004）, clinical stage （X2 = 9. 586, P = 0. 002） and pathological differentiation （X2 = 20. 372, P = 0. 001 ）. Conclusion The loss expression of hMLH1 has a correlation with pancreatic carcinogenesis, differenti- ation degree, and disease progression.