Survivin、RAGE和HMGB1在乳腺癌中表达及其临床意义

Expression of Survivin, RAGE and HMGB1 gene in human breast cancer and its clinical significance

目的探讨乳腺癌组织中Survivin、RAGE和HMGBl基因表达及其临床意义。方法对50例早期（I＋Ⅱ期）、50例晚期（Ⅲ＋Ⅳ期）乳腺癌及100例对照组蜡块标本，运用Real—timePCR和荧光原位杂交技术（FISH）技术检测Survivin、RAGE和HMGBI基因表达。并分析各基因表达与乳腺癌组织的分化程度、浸润深度、淋巴结转移、TNM分期之间的关系。结果Survivin、RAGE和HMGBl基因表达荧光实时定量PCR法上调分别为62％、73％、79％，FISH法基因扩增阳性分别为78％、69％、72％。乳腺癌TNM分期、淋巴结转移与基因高表达有密切关系，Survivin基因表达与RAGE、HMGBl表达呈正相关。结论Survivin、RAGE和HMGBl基因表达对乳腺癌早期诊断和预后分析有重要指导意义。

Objective To investigate the expression of Survivin, receptor for advanced glycation end- product（RAGE） and high mobility group protein B1 （HMGB1） gene in human breast cancer and their clinical significance. Methods The expression of Survivin, RAGE and HMGB1 gene was detected by Real-time PCR technology and fluorescence in situ hybridization （FISH） technology in the following tissue samples： 50 early breast cancers, 50 advanced breast cancers, and the corresponding adjacent normal mammary tissues. The rela- tionship of their expression and several factors such as differentiation degree, invasion, lymph node metastasis and TNM stage of cancer was explored. Results The positive expression rate of Survivin, RAGE and HMGB1 gene was 62%, 73% and 79% detected by Real-time PCR technology, 78% , 69% and 72% detected by FISH tech- nology in breast cancer tissues. The overexpression of these genes was positively correlated with TNM stage and lymph node metastasis. The expression of Survivin gene was positively correlated with the expression of RAGE and HMGB1. Conclusion Overexpression of Survivin, RAGE and HMGB1 gene is of great significance in early di- agnosis and prognosis of human breast cancer.