腹腔注射阿霉素诱导实验扩张型心肌病大鼠模型的建立

The dilated cardiomyopathy model in rat induced intraperitoneal injection of adriamycin

目的建立稳定可靠的扩张型心肌病(DCM)动物模型,以供基础实验研究。方法选用雄性SD大鼠,分为正常对照组和DCM模型组,DCM组予腹腔注射阿霉素2.5mg/(kg.week),生理盐水稀释浓度为1mg/ml,正常组腹腔注射等量生理盐水。每周根据体重调整阿霉素剂量,用药6周后,检查心脏超声、血浆脑钠肽(BNP)水平、观察心肌病理切片。结果心脏超声心动图示模型组左室射血分数(LVEF)、左室短轴缩短率(FS),各项指标明显低于正常对照组(P<0.01)。模型组大鼠血浆BNP水平高于正常对照组(P<0.01)。心肌病理HE染色观察、结果符合DCM改变。结论该制造扩张型心肌病动物模型的方法成功率高,造模周期较短,结果可靠,可以做为基础研究的动物模型。

Objective Establish a stable and reliable dilated cardiomyopathy（DCM） animal models for basic experimental research.Methods Male SD rats were divided into normal group and DCM group in random.intraperitoneal injection of doxorubicin to the DCM group（2.5 mg/（kg·week））,Diluted with normal saline concentration of 1mg/ml,Intraperitoneal injection of saline to the normal group.Weekly doxorubicin dose adjustments based on body weight,medication after 6 weeks,assess echocardiography,plasma brain natriuretic peptide（BNP） levels,myocardial biopsy.Results The DCM group left ventricular ejection fraction（LVEF）,left ventricular fractional shortening（FS）,the index was significantly lower than the normal group（P0.01）,plasma BNP levels higher than the normal group（P0.01）.Myocardial HE staining,results in line with DCM pathology.Conclusion The means of dilated cardiomyopathy animal model a high success rate,short-cycle modeling,reliable,can be used as animal models for basic research.