乙肝病毒基因组1896位点变异株与野生株的快速检测

The Rapid Qualitative Detection of Hepatitis B Virus Genome 1896 Site Variants and Wild Strains

目的建立一种能同时检测乙肝病毒基因组1896位点变异株与野生株的快速检测方法。方法设计5条特异性引物和1条特异性探针,分别克隆出含变异株与野生株的质粒作标准模板,利用荧光定量PCR检测乙肝病毒基因组1896位点变异株与野生株。结果本次实验共检测30个标本,有7个标本只检测到野生株,4个标本只检测到变异株,其余19个标本是变异株与野生株混合感染。结论本法是可行的能同时检测乙肝病毒基因组1896位点变异株与野生株的快速方法。

Objective To establish the rapid qualitative detection method of hepatitis B virus genome 1896 site variants and wild strains.Methods 5 strips of specific primers and 1 strip of specific probe were designed.Variant strain and wild strain of plasmid as standard template Were cloned,Variant strains and wild strains HBV DNA templates extracted from the serum samples were amplified by fluorescence quantitative PCR.Results The experiments detected 30 samples,Wild strains were detected in 7 samples,but no variant strains.Variant strains were detected in 4 samples,but no wild strains.Variant and wild strains of mixed infection were detected in the remaining 19 samples.Conclusion This is a feasible and rapid method to detect hepatitis B virus genome 1896 site variant and wild strains of qualitative methods.