氧化乐果对小鼠睾丸Sertoli细胞DNA的损伤作用

Sertoli cell DNA damage induced by omethoate in mice

目的研究有机磷农药氧化乐果对小鼠睾丸Sertoli细胞DNA的损伤作用。方法将40只清洁级昆明雄性小鼠随机分为对照(蒸馏水)组和1.0、2.0、4.0 mg/kg氧化乐果染毒组,每组10只。采用灌胃方式进行染毒,染毒容量为20 ml/kg,每天1次,连续染毒14 d。采用单细胞凝胶电泳技术检测小鼠睾丸Sertoli细胞的DNA损伤情况。结果与对照组比较,2.0、4.0 mg/kg氧化乐果染毒组彗星长度均明显增加,各剂量氧化乐果染毒组彗星尾长、Olive尾矩、尾长/头长、尾部DNA含量也均升高,差异有统计学意义(P<0.05)。且随着氧化乐果染毒剂量的升高,各指标均呈上升趋势。结论氧化乐果对小鼠睾丸Sertoli细胞DNA有明显的损伤作用。

Objective To explore the omethoate-induced Sertoli cell DNA damage in mice. Methods A total of 40 male Kunming mice （cleaning level） were randomly divided into four groups including the dose of 1.0, 2.0, 4.0 mg/kg body weight omethote and the control group （distilled water）, ten in each. Omethoate and distilled water were given through gavage, 20 ml/kg body weight,once a day, for 14 consecutive days. The DNA damage of the Sertoli cell induced by omethoate was detected by single-cell gel electrophoresis test （SCGE）. Results Compared with the control group, the lengths of comet of 2.0,4.0 mg/kg omethoate group increased significantly. The tail lengths, Olive tail moments, the ratios of tail length and head length, percentage of tail DNA of every omethote group increased obviously compared with those of the control group （P〈0.05）. And as the omethoate dose increased, the length of comet, tail length, Olive tail moment, the ration of tail length and head length, percentage of tail DNA appeared an upward trend. Conclusion Omethoate may cause damage of Sertoli cell DNA in mice.