裸小鼠原位接种肝癌经瘤体内蓖麻蛋白注射后疗效及其骨髓抑制的研究

EFFICACY OF INTRA-TUMOR INJECTION OF RICIN IN TREATING TRANSPLANTED HEPATOCARCINOMA

目的 为了观察蓖麻蛋白在介入治疗肝癌中的疗效 ,比较蓖麻蛋白与丝裂霉素对骨髓抑制的差别。方法 用LC 412移植瘤瘤块 ,植入实验裸鼠肝内建立裸鼠原位肝癌模型 ,以瘤体内注入生理盐水和碘油水乳剂作对照 ,比较蓖麻蛋白、碘油乳化蓖麻蛋白、丝裂霉素、在肝癌瘤体内注射后的疗效 ,观察肿瘤生长抑制率、甲胎、血像及骨髓抑制。结果 蓖麻蛋白、碘油乳化蓖麻蛋白、丝裂霉素组肿瘤生长受到显著抑制 (P <0 0 5 ) ,肿瘤组织发生不同程度坏死 ,甲胎蛋白降低与对照组比较有显著差别 (P <0 0 5 ) ,但 3种剂型间无显著性差别。周围血像及骨髓抑制丝裂霉素比蓖麻蛋白明显 (P <0 0 1)。结论 蓖麻蛋白对肝癌的治疗作用明显 ,其乳剂抗癌作用不变 ,为蓖麻蛋白瘤内注射及乳化后肝动脉导向治疗肝癌提供了实验依据。蓖麻蛋白骨髓抑制毒性明显低于丝裂霉素 。

Objective To observe the therapeutic effect of ricin by injected into transplanted human hepatocarcinoma in nude mice and the inhibition effect of bone marrow in compare with mitomycin C. Methods Human hepatocarcinoma cell line LC412 was transplanted subcutaneously into nude mice. Efficacy of intratumor injection of ricin, lipiodol emulsified ricin, and mitomycin C was tested. Saline and lipiodol were used as control. Results LC412 growth was inhibited and AFP level was decreased separately by ricin,lipiodolricin and mitomycin C (P<005), but there was no statistical difference between these three groups. Inhibition of bone marrow and peripheral blood by mitomycin C was more obvious than ricin(P<001). Conclusion An experimental therapeutic effect of ricin on hepatocarcinoma is obvious, the lipiodol emulsified ricin still remains its anticancer activity, and thus provides experimental basis for clinical intratumor injection and for hepatic arterial targeting therapy. The toxicity of ricin on bone marrow is markedly lower than mitomycin C, and thus can be administered repeatedly.