41例大肠癌组织APC基因突变检测

DETECTION OF APC GENE MUTATIONS IN 41 CASES OF COLORECTAL CANCER

目的 探讨散发性大肠癌与APC基因突变的关系。方法 41例大肠癌组织标本 ,应用银染 单链构相多态性技术分析APC基因突变情况。结果 41例大肠癌患者组织标本中 ,APC基因突变率为 48 8% ( 2 0 41例 )。引物 1、引物 2组织标本扩增产物突变检出率分别为 15 41( 36 6% )、7 41( 17 1% ) ,两者同时检测到突变的有 2例。结果还表明 ,APC基因的突变与CEA水平、淋巴结转移、病理分化程度统计学上没有显著性关系。结论 APC基因突变是散发性大肠癌较常见的基因改变事件。进行多位点突变检测可以提高突变的检出率。实验还表明 ,APC基因突变可能是大肠癌发生。

Objective To study the relationships between sporadic colorectal cancer and APC gene mutations. Methods APC suppressor gene mutations were detected by two primers designed. Mutational examinations of APC suppressor in the cancer tissues of 41 patients with sporadic colorectal cancer were investigated by silverstain polymerase chain reactionsingle strand conformation polymorphism (PCRSSCP). Results Of 41 cancer speimens mutations were detected in 15 cases (36.3 %) by the primer 1 and in 7 cases (17.1 %) by the primer2,and 2 cases showed both mutations. Conclusion APC gene mutations are common alterations in sporadic colorectal cancer. But the positive rate of detection was not so satisfactory, and a combine detection of multiple points may be helpful. This paper also showed that there were no relationships between APC gene mutations are common alterations in sporadic colorectal cancer. But the positive rate of detection was not so satisfactory, and a combined detection of multiple points may be helpful. This paper also showed that there were no relationships between APC gene mutations and Dukes' staging, lymphatic metastasis as well as CEA level. $$$$