神经干细胞移植对大鼠慢性脊髓损伤的疗效及对EphA2的影响

Effect of neural stem cells on EphA2 expression in chronic compression spinal cord injury in rats

目的研究神经干细胞对于慢性脊髓损伤的治疗性作用及其机制。方法用SD大鼠100只分为假手术组(10只),轻度模型组(10只),轻度治疗组(20只),中度模型组(10只),中度治疗组(20只),重度模型组(10只)和重度治疗组(20只),治疗组均进行神经干细胞注射治疗。使用大鼠后肢运动评分进行大鼠运动功能检测,所取脊髓标本进行原位杂交分析,对脊髓肝癌细胞株受体A2(EphA2)阳性细胞率进行统计分析。结果和假手术组相比,脊髓损伤后,不论模型组还是治疗组,大鼠的后肢运动能力均出现了显著性的下降,治疗组的恢复速度要明显快于模型组。假手术组有几乎无EphA2阳性细胞,模型组EphA2阳性细胞明显升高(P<0.01),中度压迫组及重度压迫组较轻度压迫组升高(P<0.05),中度压迫组及重度压迫组间差异无统计学意义(P>0.05)。治疗组EphA2阳性细胞均高于对应模型组。结论神经干细胞移植可升高EphA2水平,尤其对重度压迫患者,有助于行为学上的恢复。

【Objective】 To investigate the effect of neural stem cells on EphA2 expression in chronic compression spinal cord injury rats.【Methods】 100 SD rats were divided into sham operation group（n =10）,mild model group（n =10）,mild treated group（n =20）,moderate model group（n =10）,moderate treated group（n =20）,severe model group（n =10） and severe treated group（n =20）,the treated group were treated with the neural stem cells injection.The rats＇ motor function were tested by the rat hind limb motor scores and the erythropoietin-producing hepatoma line A2（EphA2） expression were observed by in situ hybridization.【Results】 Compared with sham group,the rats hindlimb motor ability were significant declined in treatment groups and model groups.The recovery speed in the treated groups was faster than that in the model groups.The number of EphA2 positive cells were significantly higher in model groups than in sham operation group（P 0.01）,higher in moderate and severe injury groups than in mild injury group（P 0.05）,but no difference was found between moderate and severe injury groups（P 0.05）,and higher in treated group than corresponding model group.【Conclusions】 Neural stem cell in injury location could help SCI recovery and increase the expression of EphA2.