枇杷胚性培养物中2个ACS基因的克隆及生物信息学分析

Cloning and Bioinformatics Analysis of Two ACS Genes from Embryonic Cultures in Eriobotrya japonica

以枇杷(Er iobotrya japonica)胚性培养物为材料,采用同源克隆法分离2个ACC合成酶(1-aminocyclopropane-1-carboxylic acid synthase,简称ACS)基因家族成员EjACS-1和EjACS-2,GenBank登录号分别为GQ370519和GQ370520。结果显示:EjACS-1和EjACS-2的开放阅读框(ORF)长度均为1 464 bp,编码487个氨基酸。2个ACS基因之间核苷酸和氨基酸的一致性分别为92%和93%,且枇杷ACS与蔷薇科苹果亚科植物多种ACS高度同源。此外,从枇杷基因组DNA中分离了1个含有3个内含子、长度2 319 bp的基因gACS,GenBank登录号为GU180353。EjACS-1和EjACS-2的克隆,为今后研究枇杷组织培养中乙烯的作用机理奠定基础。

Using homologous cloning method, two members of 1-aminocyclopropane-l-carboxylic acid synthase （ACS）gene family were isolated from Eriobotrya japonica embryonic cultures, and named as EjACS-1 and EjACS-2. Both genes had been submitted to the GenBank database, the accession numbers were GQ370519 and GQ370520, respectively. The length of EjACS-I and EjA CS-20RF was both 1 464 bp, which encoding 487 amino acids. The identities of nucleotide and amino acid between two A CS genes were 92% and 93%, respectively. Also, EjACS-1 and EjACS-2 were highly homologous to various ACS in other Rosaceae apple subfamilies plants. Moreover, gACS which contained 3 introns was obtained from the genomic DNA in loquat. The length of gACS was 2319 bp, and the accession number was GU180353 in GenBank. The cloning of EjACS-1 and EjACS-2 could provide the foundation for further study the mechanism of ethylene during in vitro cultures of Eriobotrya japonica.