从6例血友病患者分离的TT病毒基因克隆及序列测定

Cloning and sequencing of transfusion transmitted viruses(TTV) isolated from 6 patients with hemophilia

目的 了解血友病人的输血传播病毒 (TTV)基因型及其在个体内存在状况。方法 对6例血友病人血清中分离的TTV进行基因克隆 ,从每例病人的标本随机选多个克隆进行测序 ,比较其核苷酸和氨基酸序列。结果 除 1例病人的 5个克隆TTVDNA序列基本相同外 ,其余病人所测的TTVDNA克隆序列均不完全相同。根据同源性分析 ,该 6例病人的TTVDNA主要可分为 3组 :G1a、G1b和G2基因型。结论 同一病人可混合感染不同TTV毒株。

Objective To study genotypes and status TTV existence in patients with hemophilia. Method TTV DNA isolated from sera of 6 hemophilic patients by polymerase chain reaction(PCR) were cloned using TA easy vector. 3 5 clones selected from each patient randomly were sequenced, and then compared with TTV DNA sequences in GenBank. Results Five of 6 patients have different sequences of TTV DNA clones, and only one patient has the same genotype of the virus. All the clones obtained from 6 patients can be divided into 3 groups:G1a,G1b and G2. Conclusion Mixed infection of different TTV strains was found in patients with hemophilia.