依达拉奉对海人酸致颞叶癫痫大鼠海马神经元的保护作用

The protective effect of edaravone on hippocampal neurons in kainic acid-induced epileptic rats

目的本实验观察依达拉奉对海人酸致痫大鼠海马神经元损伤的保护作用。方法选用成年健康雄性Wistar大鼠18只,体重260±20g。实验动物随机分为3组,①sham组(n=6):右侧海马CA3区注入等量的生理盐水;②KA模型组(n=6):右侧海马CA3区注入KA 4μg.kg-1(4μg/μl);③依达拉奉组(n=6):右侧海马CA3区注入KA 4μg.kg-1(4μg/μl)后,即刻给予依达拉奉10mg.kg-1.d-1腹腔注射。于大鼠注药或假手术后立即观察各组大鼠的行为学表现,于7d断头取脑,石蜡切片进行硫堇染色,于光学显微镜下观察注药对侧(左侧)海马CA1、CA3区及CA4门区组织形态学特征,并对其进行组织学分级。结果 Sham组大鼠注射对侧海马CA1、CA3和CA4门区无明显组织损伤,组织学分级多为0～1级,ND值为198±20.62和212±30.14;模型组KA致痫大鼠可见明显的组织损伤,组织学分级多为2～3级,ND值为79±13.72和90±14.98,与sham组相比,组织学分级显著升高(p<0.05),ND值显著降低(p<0.01)。依达拉奉组大鼠海马CA1区可见少量、散在性神经元坏死,组织学分级多1～2级,ND值为101±16.85和135±12.17。与模型组相比,组织学分级降低(p<0.05),ND值显著升高(p<0.05)。结论依达拉奉能够减轻KA致痫大鼠海马神经元的损伤,对神经元具有保护作用。

Objective To investigate the protective effect of edaravone on hippocampal neurons in kainic acid - induced epileptic rats. Methods Fifty - one mature male Wistar rats （ weight 260 ± 20g ） were randomly divided into 3 groups ： ①sham operation group （ n = 6 ） ： administrated 0.9% saline the same volume as model group; ② KA model group （n = 6）： administrated kainic acid （4μg/kg） in the right hippocampal CA3 region with sereotaetic technique; ③edaravone group （n = 6） ： administrated kainic acid （4μg/kg） in the right hippocampus CA3 region with sereotaetic technique, and immediately administrated edaravone （10 mg/ kg） once dayly i. p. All rats were sacrificed on the seven day after the injection of saline or kainic acid. Histological changes of the CA1 and CA3 regions of the hippocampus were observed under thionine staining. Histological changes were divided into the following 4 grades （ histological grade, HG） under light microscope. Results No significant neuronal damage was found in contralateral hippocampus of rats in the sham group. The HD in the CA1 ,CA3 was 0 - 1 ,and values of neuronal density （ND） were 198 ±20.62, 212 ± 30. 14. In model group , compared with the sham group, the values of ND was 79± 13.72, 90 ± 14.98, significantly higher （ P 〈 0.01 ）, HG （ grade 2 - 3 ） was much lower （ P 〈 0.05）. In edaravone group , compared with the model group, there were scatted necrosis cells in the CA1, CA3 , and values of ND was 121 ± 16.85, 145± 12.17, significantly lower （P 〈0.01 ）. HG （gradel -2） was much higher（P 〈0.05）. Conclusion Edaravone can protect hippocampal neurons from damage in kainie acid -induced epileptic rats.