不同饲养环境对局灶性脑梗死大鼠行为学恢复的影响

Different environmental intervention effect on behavioral recovery in rats after unilateral local cerebral infarction

目的观察不同环境设置对局灶性脑梗死大鼠行为学恢复的影响,为临床应用丰富环境、探索学习等不同环境进行脑卒中康复治疗提供基础理论支持和实验依据。方法将实验动物随机分为假手术对照组(15只)和大脑中动脉阻塞(MCAO)(90只)。手术组用电凝法造成右侧MCAO模型。MCAO组大鼠于MCAO手术后,再随机分为社会交往组(30只)、探索学习组(30只)和丰富环境组(30只)。社会交往组5只一组,群居于标准笼;探索学习组10只一组,居于迷宫笼;丰富环境组30只居于丰富环境笼。鼠MCAO造模成功后各实验组随机取10只分别于1d、3d、7d、14d、28d时和假手术组随机选取10只分别于术后1d、3d时进行Berderson神经功能评分和修订的神经功能评分(mNSS)评价。各MCAO组分别于术后1d、3d、7d、14d、28d随机选取5只以及假手术组于术后1d、3d、7d随机选取5只大鼠处死,剖颅取脑,石蜡包埋,切片,HE染色。结果行为学评分:Berderson神经功能评分,丰富环境组及探索学习组于MCAO后14和28d时恢复明显优于社交组(p<0.05),各时间点丰富环境组和探索学习组之间比较无明显差异(p>0.05);修订的神经功能评分(mNSS),丰富环境组和探索学习组于MCAO后14、28d mNSS运动和平衡单项评分均明显低于社交组(p<0.05),而总分则从7d开始明显低于社交组。

Objective The experiment intended to observe the effects of the different environmental intervention on the behavioral recovery in order to offer the basic theoretical support and experimental instruction for stroke rehabilitation. Methods One hundred and five male SD rats,3 -4 months,weight 240 -260g,were divided into sham （n =15） and MCAO（middle cerebral artery occlusion） groups（n =90）. The rats in MCAO group were subjected to electric coagulation of MCA and then were assigned randomly to social commun - ication group（ n = 30, 5 as a group housed in a standard cage）＇, learn training group（n =30, 10 as a group housed in a maze cage） , rich environment group（n = 30 , housed in a rich environment cage）. 10 rats were randomly chosen from each operated group after MCAO at ld, 3d- 7d, 14d, and 28d and 10 rats from sham group at ld, 3d, for Berderson test and mNSS test . 5 rats from each operated group at ld, 3d, 7d, 14d, and 28d after MCAO and 5 rats from sham group at ld, 3d, 7dwere randomly assigned to be killed . The expression of VEGF in peri -ischemic cortex were examined from paraffin slice and immunohistochemistry staining. Results The score of behavioral test ： Berderson test： The scores in rich environment and learn training groups on dayl4and 28 were better than those in social communication groups（p 〈0.05 ）. There was no significant difference in the score between rich environment and learn training groups at any time point. （p 〉0.05） mNSS test： the scores for motor ability and beam balance on day 14 and 28, were lower than that in social communication groups （p 〈 0. 05 ） and for total score in rich environment and learn training groups were significantly lower than that in social communication groups（p 〈 0.05 ）afte 7d. Conclusion The up - regulation may contribute to the improvement of the recovery of extremity functions resulted from learn training and the enriched environment.