vimentin在三阴性乳腺癌中的表达及临床意义

Expression of vimentin in triple negative breast cancer and its clinical significance

目的研究波形蛋白(vimentin)在人三阴性乳腺癌中的表达及意义。方法采用免疫组化和Westernblot检测122例三阴性人乳腺癌组织中vimentin表达,并分析其与临床病理特征及预后的关系;Westernblot分析三阴性乳腺癌细胞系MDA-MB-231与非三阴性乳腺癌细胞系MCF-7中vimentin的表达;Transwell侵袭试验检测细胞的侵袭能力。结果在122例三阴性人乳腺癌组织中vimentin表达明显高于对应的癌旁组织(P<0.05),同时vimentin的表达与其病理分化程度存在明显的相关性(P<0.05)。vimentin在三阴性乳腺癌细胞MDA-MB-231中呈高表达,而在非三阴性的MCF-7细胞中表达较低。结论三阴性乳腺癌细胞中vimentin表达增高,vimentin的表达可能与三阴性乳腺癌的侵袭和迁移有关。

Objective To study vimentin expression in human triple negative breast cancer（TNBC）and its clinical significance. Methods The expression of vimentin in 122 triple negative human breast cancer tissues were detected by using immunohistochemistry and western blot, and the relationship between clinical and pathological data was analyzed. The cell invasion ability was analyzed by transwell invasion assay. Results vimentin expression in 122 cases of triple negative human breast cancer tissues was significantly higher than that in the adjacent tissues （P0.05）. There was obvious correlation between the expression of vimentin and pathologic differentiation （P0.05）. Vimentin was highly expressed in TNBC cells MDA-MB-231,while it was lowly expressed in the non-TNBC MCF-7 cells. Conclusions Increased vimentin expression in TNBC cells may be associated with the TNBC invasion and migration.