蜈蚣败毒饮对人脐静脉内皮细胞增殖的影响

The Experimental Study of Centipede Antiphlogistic Decoction on Proliferation of Human Umbilical Vein Endothelial Cells

目的研究蜈蚣败毒饮对HUVEC增殖的影响,探讨其对银屑病的作用机制。方法采用随机分组,将实验动物分为6组,分别制备含药血清。设空白组、甲氨蝶呤片组、复方青黛胶囊组为对照组;设蜈蚣败毒饮低、中、高剂量含药组为实验组。研究应用血清药理学技术,采用四唑盐(MTT)比色实验测定蜈蚣败毒饮对HUVEC增殖的影响。结果中药高剂量组HUVEC增殖率明显低于中药对照组。中药低剂量组、中药中剂量组HUVEC增殖率与中药对照组比较无统计学差异。结论蜈蚣败毒饮可抑制HUVEC增殖。

Objective： To explore the effect of centipede antiphlogistic decoction on the proliferation of HUVEC,and to discuss its mechanism on psoriasis.Methods： Experimental animals were randomly divided into six groups,respectively preparing medicated serum.blank group,methotrexate tablets,compound Qingdai capsule group were set to be control groups;Centipede antiphlogistic decoction in low,medium and high doses drug-containing group were set to be the experimental groups.The influence of centipede antiphlogistic decoction on the proliferation of HUVEC was determined with the serum pharmacology technology and MTT assay.Results： Chinese medicine in high dose group of HUVEC proliferation was significantly lower than that of control group.Chinese medicine in low dose group,middle dose group on HUVEC proliferation rate was no Statistical significance compared with that of control group.Conclusion： The centipede antiphlogistic decoction can inhibit the proliferation of HUVEC.